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KMID : 1094720070120030302
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 3 p.302 ~ p.307
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Statistical optimization of medium components for the production of xylanase by Aspergillus niger KK2 in submerged cultivation
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Min Byeong-Jo
Park Yang-Soon
Kang Seong-Woo
Song Yoon-Seok
Lee Jong-Ho
Park Chul-Hwan
Kim Chan-Wha
Kim Seung-Wook
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Abstract
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Medium composition was optimized for the production of xylanase byAspergillus niger KK2 using statistical experimental designs. Corn steep liquor (CSL) and industrial yeast extract (IYE) were the most important factors affecting xylanase activity. The medium that produced the optimum conditions for the production of xylanase contained 3% rice straw, 1% wheat bran, 6.3% CSL, 0.15% IYE, and 0.5% KH2PO4. After 4 days of cultivation under optimized conditions in a 2.5-L stirred tank reactor the activity and productivity of xylanase were 620 IU/mL and 6,458 IU/L.h, respectively. The highest xylanase activity obtained using the optimized medium was 80% greater than the activity obtained using basal medium. The xylanase activity predicted by a polynomial model was 670 IU/ml.
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KEYWORD
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Aspergillus niger, corn steep liquor (CSL), response surface methodology (RSM), rice straw, xylanase
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